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clinimacs cd141 bdca 3 biotin microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec clinimacs cd141 bdca 3 biotin microbeads
    Clinimacs Cd141 Bdca 3 Biotin Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd141+biotin/CD141+(BDCA-3)+Antibody%2C+anti-mouse%2C+REAfinity/pm38212127-81-37-48
    Average 92 stars, based on 4 article reviews
    clinimacs cd141 bdca 3 biotin microbeads - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody
    Article Snippet: Flow Cytometry PBMC samples were stained and analyzed by flow cytometry on a BD LSRII (BD Biosciences, San Jose, CA) using FlowJo analysis software (Treestar, Ashland, OR) as previously described ( 14 ). .. The following monoclonal antibodies were used in this study: CD1c-PE (AD5-8F7, Miltenyi Biotec, Auburn, CA), CD3-PE-Cy5.5 (S4.1, Invitrogen, Carlsbad, CA), CD4-APC-Alexa Fluor 750 (RPA-T4, eBioscience, San Diego, CA), CD4-Qdot655 (S3.5, Invitrogen), CD11c-PE-Cy7 (3.9, Biolegend, San Diego, CA), CD14-Alexa Fluor 700 (M5E2, BD Biosciences, San Jose, CA), CD14-Qdot800 (TüK4, Invitrogen), CD16-PerCp-Cy5.5 (3G8, BD Biosciences), CD16-PE-TexasRed (3G8, Invitrogen), CD19-PerCp-Cy5.5 (SJ25C1, BD Biosciences), CD34-PerCp-Cy5.5 (8G12, BD Biosciences), CD40-APC-H7 (5C3, BD Biosciences), CD86-Pacific Blue (IT2.2, Biolegend), CD141-biotin (AD5-14H12, Miltenyi Biotec), CD303-APC (AC144, Miltenyi Biotec), HLA-DR-Qdot605 (Tü36, Invitrogen). .. Streptavidin-Pacific Orange and Streptavidin-Qdot585 (Molecular Probes/Invitrogen, Carlsbad, CA) were used as secondary staining reagent for CD141-biotin.

    Recombinase Polymerase Amplification:

    Article Title: Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody
    Article Snippet: Flow Cytometry PBMC samples were stained and analyzed by flow cytometry on a BD LSRII (BD Biosciences, San Jose, CA) using FlowJo analysis software (Treestar, Ashland, OR) as previously described ( 14 ). .. The following monoclonal antibodies were used in this study: CD1c-PE (AD5-8F7, Miltenyi Biotec, Auburn, CA), CD3-PE-Cy5.5 (S4.1, Invitrogen, Carlsbad, CA), CD4-APC-Alexa Fluor 750 (RPA-T4, eBioscience, San Diego, CA), CD4-Qdot655 (S3.5, Invitrogen), CD11c-PE-Cy7 (3.9, Biolegend, San Diego, CA), CD14-Alexa Fluor 700 (M5E2, BD Biosciences, San Jose, CA), CD14-Qdot800 (TüK4, Invitrogen), CD16-PerCp-Cy5.5 (3G8, BD Biosciences), CD16-PE-TexasRed (3G8, Invitrogen), CD19-PerCp-Cy5.5 (SJ25C1, BD Biosciences), CD34-PerCp-Cy5.5 (8G12, BD Biosciences), CD40-APC-H7 (5C3, BD Biosciences), CD86-Pacific Blue (IT2.2, Biolegend), CD141-biotin (AD5-14H12, Miltenyi Biotec), CD303-APC (AC144, Miltenyi Biotec), HLA-DR-Qdot605 (Tü36, Invitrogen). .. Streptavidin-Pacific Orange and Streptavidin-Qdot585 (Molecular Probes/Invitrogen, Carlsbad, CA) were used as secondary staining reagent for CD141-biotin.

    Staining:

    Article Title: Human blood dendritic cell subsets exhibit discriminative pattern recognition receptor profiles
    Article Snippet: Unconjugated antibodies against PRRs were detected using either rabbit anti-mouse PE (Dako Cytomation, Glostrup, Denmark) or goat anti-rabbit FITC (BD Biosciences, San Jose, CA). .. To identify DC subsets, samples were additionally stained with an antibody cocktail including CD1c-PE, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-Peridinin chlorophyll protein (PerCp)-Cy5.5 (Biolegend, San Diego, CA), CD3-allophycocyanin (APC), CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA) or a cocktail including CD123-biotin, CD16-PE-Cy7 (BD Biosciences), HLA-DR-PerCp-Cy5.5, CD3-PE (BD Biosciences), CD14-PE and CD19-PE (Dako). .. The PRRs targeted with PE-conjugated antibodies were instead stained with antibody cocktails including HLA-DR-PerCp-Cy5.5 and either CD123-biotin, CD16-PE-Cy7, CD3-FITC (BD Biosciences), CD14-FITC (Invitrogen) and CD19-FITC (Dako) or CD1c-FITC, CD141-biotin, CD3-APC, CD14-APC and CD19-APC.



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    (A). Specific DC subsets were identified as HLA-DR positive, lineage negative cells that expressed either <t>CD141,</t> CD1c, CD16 or CD123, using flow cytometric analysis of viable cells in the FSC-SSC gate. (B). Flow cytometric analysis was performed on non-allergic controls (n-a) (n = 6) as well as on AIT subjects (n = 7) before treatment (t0), as well as after 8 weeks (t8w) and 1 year (t1y), to determine the frequencies of CD141 + , CD1c + , CD123 + and CD16 + DC subsets. Plots show each donor value as well as mean values and SD variations. * = p<0.05, Friedman’s test followed by a Dunn's multiple comparison test.
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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes

    doi: 10.1016/j.celrep.2020.108337

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Mouse anti human CD141 biotin, clone AD5–14H12 , Miltenyi Biotec , RRID:AB_2726093; Miltenyi Biotec Cat# 130-113-316.

    Techniques: Recombinant, DNA Library Preparation, Gene Expression, Software

    (A). Specific DC subsets were identified as HLA-DR positive, lineage negative cells that expressed either CD141, CD1c, CD16 or CD123, using flow cytometric analysis of viable cells in the FSC-SSC gate. (B). Flow cytometric analysis was performed on non-allergic controls (n-a) (n = 6) as well as on AIT subjects (n = 7) before treatment (t0), as well as after 8 weeks (t8w) and 1 year (t1y), to determine the frequencies of CD141 + , CD1c + , CD123 + and CD16 + DC subsets. Plots show each donor value as well as mean values and SD variations. * = p<0.05, Friedman’s test followed by a Dunn's multiple comparison test.

    Journal: PLoS ONE

    Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

    doi: 10.1371/journal.pone.0148838

    Figure Lengend Snippet: (A). Specific DC subsets were identified as HLA-DR positive, lineage negative cells that expressed either CD141, CD1c, CD16 or CD123, using flow cytometric analysis of viable cells in the FSC-SSC gate. (B). Flow cytometric analysis was performed on non-allergic controls (n-a) (n = 6) as well as on AIT subjects (n = 7) before treatment (t0), as well as after 8 weeks (t8w) and 1 year (t1y), to determine the frequencies of CD141 + , CD1c + , CD123 + and CD16 + DC subsets. Plots show each donor value as well as mean values and SD variations. * = p<0.05, Friedman’s test followed by a Dunn's multiple comparison test.

    Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

    Techniques: Comparison

    FcR expression based on flow cytometric analysis on cells from non-allergic controls (n-a) (n = 6) and AIT donors at t0, t8w and t1y (n = 7, with the exception of FcεRI on CD141 + DCs (n = 2) and CD1c + DCs (n = 6)). Plots show each donor value as well as mean values and SD variations. * = p<0.05; ** = p<0.01, (Friedman’s test followed by a Dunn's multiple comparison test. FcRs altered significantly during AIT are framed. FcεRI on CD141 + DCs not tested for significance, too few AIT samples).

    Journal: PLoS ONE

    Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

    doi: 10.1371/journal.pone.0148838

    Figure Lengend Snippet: FcR expression based on flow cytometric analysis on cells from non-allergic controls (n-a) (n = 6) and AIT donors at t0, t8w and t1y (n = 7, with the exception of FcεRI on CD141 + DCs (n = 2) and CD1c + DCs (n = 6)). Plots show each donor value as well as mean values and SD variations. * = p<0.05; ** = p<0.01, (Friedman’s test followed by a Dunn's multiple comparison test. FcRs altered significantly during AIT are framed. FcεRI on CD141 + DCs not tested for significance, too few AIT samples).

    Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

    Techniques: Expressing, Comparison

    Flow cytometric analysis was performed on CD141 + DCs from non-allergic donors (n = 6), as well as allergic donors (n = 5) at t0, t8w and t1y. Plots show each donor value as well as mean values and SD variations. Statistical analysis of AIT donors compared to non-allergic donors was performed with a Kruskal-Wallis test followed by a Dunn's multiple comparison test. * = p<0.05. ns-non-significant; w-weeks; y-years.

    Journal: PLoS ONE

    Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

    doi: 10.1371/journal.pone.0148838

    Figure Lengend Snippet: Flow cytometric analysis was performed on CD141 + DCs from non-allergic donors (n = 6), as well as allergic donors (n = 5) at t0, t8w and t1y. Plots show each donor value as well as mean values and SD variations. Statistical analysis of AIT donors compared to non-allergic donors was performed with a Kruskal-Wallis test followed by a Dunn's multiple comparison test. * = p<0.05. ns-non-significant; w-weeks; y-years.

    Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

    Techniques: Comparison